Extension of the substrate spectrum by an amino acid substitution at residue 219 in the Citrobacter freundii cephalosporinase
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چکیده
منابع مشابه
Kinetic studies on inactivation of Citrobacter freundii cephalosporinase by sulbactam.
The inactivation kinetics for inhibition by sulbactam (CP45,899) of Citrobacter freundii GN346 cephalosporinase were studied in detail and compared with those of type Ib penicillinase or TEM-2 beta-lactamase mediated by R plasmid RGN823. The rate constant for progressive inactivation of the cephalosporinase was significantly larger than that measured with the penicillinase. The number of sulbac...
متن کاملCovalent binding of moxalactam to cephalosporinase of Citrobacter freundii.
The inhibition of Citrobacter freundii cephalosporinase activity by moxalactam is shown to be due to the formation of a transiently stable covalent complex, probably acyl enzyme. The covalent complex formed was identified by coelution of [14C] moxalactam with the enzyme by using Sephadex G-25 gel filtration in the presence of 5.7 M guanidine hydrochloride and by analytical isoelectric focusing....
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The interaction of cefpirome and a cephalosporinase from Citrobacter freundii, including hydrolysis and inhibition, was studied in comparison with those of cefotiam, cefotaxime, and ceftazidime. Cefpirome was hydrolyzed by the enzyme more rapidly at Vmax than were cefotaxime and ceftazidime. However, the low affinity of the enzyme for cefpirome caused a reduction in the hydrolytic rate of cefpi...
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this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...
a study of the fifth child and ben in the world by doris lessing in the light of julia kristevas psychoanalytic concepts
این مطالعه به بررسی عوامل روانشناختی کریستوادردو رمان دوربس لسینگ،فرزندبنجم و بن دردنیای واقعی می بردازد.موفقیت یا شکست کاراکترهادر تکمیل شکست تتیزی یه کمک بدر وهم از مهم ترین دغدغه محقق می باشد.به بررسی تحلیل روانشناختی تمامی کاراکترها خصوصا بن برداخته و به دنبال نشانه هایی از جامعه شیشه ای کریستوا می باشد.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1990
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.172.8.4348-4351.1990